Biophysical and functional study of CRL5^Ozz, a muscle specific ubiquitin ligase complex.

ABSTRACT: Ozz, a member of the SOCS-box family of proteins, is the substrate-binding component of CRL5^Ozz, a muscle-specific Cullin-RING ubiquitin ligase complex composed of Elongin B/C, Cullin 5 and Rbx1. CRL5^Ozz targets for proteasomal degradation selected pools of substrates, including sarcolemma-associated β-catenin, sarcomeric MyHC_emb and Alix/PDCD6IP, which all interact with the actin cytoskeleton. Ubiquitination and degradation of these substrates are required for the remodeling of the contractile sarcomeric apparatus. However, how CRL5^Ozz assembles into an active E3 complex and interacts with its substrates remain unexplored. Here, we applied a baculovirus-based expression system to produce large quantities of two subcomplexes, Ozz-EloBC and Cul5-R

SUBMITTER: Campos Y 

PROVIDER: S-EPMC9098882 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature