A Multicomponent Polymer-Metal-Enzyme System as Electrochemical Biosensor for H₂O₂ Detection.

ABSTRACT: Herein, an Au nanoparticles-polydopamine-poly acrylic acid-graphene (Au NPs-PDA-PAA-graphene) multicomponent nanohybrid is fabricated by surface functionalization of graphene alongside extensive in-situ growth of Au nanoparticles. The as-obtained nanocomposite possesses good hydrophilicity, excellent biocompatibility and high biomolecules loading capacity, which acts as an ideal platform for enzyme modification. Considering this fact, Horseradish peroxidase is expressively immobilized upon Au NPs-PDA-PAA-graphene surface, in order to lay the foundations of a biosensor that is majorly based on enzymatic activity. The biosensor exhibits higher sensitivity towards the determination of H₂O₂ with linearity ranging from 0.1 μm upto 20 mm, and the limit of detection g

SUBMITTER: Tong P 

PROVIDER: S-EPMC9099068 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature