Effect of Sample Transportation on the Proteome of Human Circulating Blood Extracellular Vesicles.

ABSTRACT: Circulating extracellular vesicles (cEV) are released by many kinds of cells and play an important role in cellular communication, signaling, inflammation modulation, coagulation, and tumor growth. cEV are of growing interest, not only as biomarkers, but also as potential treatment targets. However, very little is known about the effect of transporting biological samples from the clinical ward to the diagnostic laboratory, notably on the protein composition. Pneumatic tube systems (PTS) and human carriers (C) are both routinely used for transport, subjecting the samples to different ranges of mechanical forces. We therefore investigated qualitatively and quantitatively the effect of transport by C and PTS on the human cEV proteome and particle size distribution. We found that samples trans

SUBMITTER: Uldry AC 

PROVIDER: S-EPMC9099550 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature