Automated Data Generation for Raman Spectroscopy Calibrations in Multi-Parallel Mini Bioreactors.

ABSTRACT: Raman spectroscopy is an analytical technology for the simultaneous measurement of important process parameters, such as concentrations of nutrients, metabolites, and product titer in mammalian cell culture. The majority of published Raman studies have concentrated on using the technique for the monitoring and control of bioreactors at pilot and manufacturing scales. This research presents a novel approach to generating Raman models using a high-throughput 250 mL mini bioreactor system with the following two integrated analysis modules: a prototype flow cell enabling on-line Raman measurements and a bioanalyzer to generate reference measurements without a significant time-shift, compared to the corresponding Raman measurement. Therefore, spectral variations could directly be correlated wit

SUBMITTER: Graf A 

PROVIDER: S-EPMC9099804 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature