Dataset Information

Silica Nanoparticles Enhance the Disease Resistance of Ginger to Rhizome Rot during Postharvest Storage.

ABSTRACT: Silica nanoparticles (SiNPs) offer an ecofriendly and environmentally safe alternative for plant disease management. However, the mechanisms of SiNPs-induced disease resistance are largely unknown. This research evaluated the application of SiNPs in controlling the postharvest decay of ginger rhizomes inoculated with Fusarium solani. In vitro study showed that SiNP had little inhibitory effect on mycelial growth and spore germination of F. solani and did not significantly change mycelium's MDA content and SDH activity. In vivo analysis indicated that SiNPs decreased the degree of decay around the wounds and decreased the accumulation of H₂O₂ after long-term pathogenic infection through potentiating the activities of antioxidant enzymes such as SOD, APX, PPO, and CAT. SiNP150 increased the CHI, PAL, and GLU activity at the onset of the experiment. Moreover, SiNP150 treatment increased total phenolics contents by 1.3, 1.5, and 1.2-times after 3, 5, and 7 days of treatment, and increased total flavonoids content throughout the experiment by 9.3%, 62.4%, 26.9%, 12.8%, and 60.8%, respectively. Furthermore, the expression of selected phenylpropanoid pathway-related genes was generally enhanced by SiNPs when subjected to F. solani inoculation. Together, SiNPs can effectively reduce the fungal disease of ginger rhizome through both physical and biochemical defense mechanisms.

SUBMITTER: Zhou J

PROVIDER: S-EPMC9099806 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Silica Nanoparticles Enhance the Disease Resistance of Ginger to Rhizome Rot during Postharvest Storage.

Zhou Jie J Liu Xuli X Sun Chong C Li Gang G Yang Peihua P Jia Qie Q Cai Xiaodong X Zhu Yongxing Y Yin Junliang J Liu Yiqing Y

Nanomaterials (Basel, Switzerland) 20220421 9

Silica nanoparticles (SiNPs) offer an ecofriendly and environmentally safe alternative for plant disease management. However, the mechanisms of SiNPs-induced disease resistance are largely unknown. This research evaluated the application of SiNPs in controlling the postharvest decay of ginger rhizomes inoculated with <i>Fusarium solani</i>. In vitro study showed that SiNP had little inhibitory effect on mycelial growth and spore germination of <i>F. solani</i> and did not significantly change my ...[more]

PMID: 35564127

Similar Datasets

Project description:Postharvest deterioration of ginger rhizome caused by microorganisms or wound infections causes significant economic losses. Fusarium solani is one of the important causal agents of prevalent ginger disease soft rot across the world. The massive and continuous use of chemical fungicides in postharvest preservation pose risks to human health and produce environmental contamination. Hence, new alternative tools are required to reduce postharvest deterioration and extend the postharvest life of ginger. In this study, the use of silicon nanoparticles (SiNPs) on the storability of ginger rhizomes during postharvest storage and their resistance to Fusarium solani was investigated. The results showed that 50, 100, and 150 mg L-1 of SiNPs increased the firmness of the ginger rhizome during storage but decreased the decay severity, water loss, total color difference, and the reactive oxygen species (ROS; H2O2 and superoxide anion) accumulation. Specifically, 100 mg L-1 (SiNP100) demonstrated the best effect in the extension of postharvest life and improved the quality of the ginger rhizomes. SiNP100 application increased the activities of antioxidant enzymes (SOD and CAT) and the total phenolics and flavonoid contents, thereby reducing the ROS accumulation and malondialdehyde (MDA) content. Meanwhile, SiNP100 treatment negatively impacts the peroxidase (POD) and polyphenol oxidase (PPO) activities, which may have contributed to the lower level of lignin and decreased total color difference. SiNP100 likely decreased water loss and the transfer of water by altering the expression of aquaporin genes. Moreover, SiNP100 modulated the expression of lignin synthesis and phytopathogenic responses genes including MYB and LysM genes. Furthermore, SiNP100 inhibited Fusarium solani by preventing the penetration of hyphae into cells, thus decreasing the severity of postharvest pathogenic decay. In summary, this study revealed the physiology and molecular mechanisms of SiNPs-induced tolerance to postharvest deterioration and resistance to disease, which provides a foundation for using SiNPs resources as a promising alternative tool to maintain ginger quality and control postharvest diseases.

Project description:BackgroundPlant-associated microorganisms can be found in various plant niches and collectively comprise the plant microbiome. The plant microbiome assemblages have been extensively studied, primarily in model species. However, a deep understanding of the microbiome assembly associated with plant health is still needed. Ginger rhizome rot has been variously attributed to multiple individual causal agents. Due to its global relevance, we used ginger and rhizome rot as a model to elucidate the metabolome-driven microbiome assembly associated with plant health.ResultsOur study thoroughly examined the biodiversity of soilborne and endophytic microbiota in healthy and diseased ginger plants, highlighting the impact of bacterial and fungal microbes on plant health and the specific metabolites contributing to a healthy microbial community. Metabarcoding allowed for an in-depth analysis of the associated microbial community. Dominant genera represented each microbial taxon at the niche level. According to linear discriminant analysis effect size, bacterial species belonging to Sphingomonas, Quadrisphaera, Methylobacterium-Methylorubrum, Bacillus, as well as the fungal genera Pseudaleuria, Lophotrichus, Pseudogymnoascus, Gymnoascus, Mortierella, and Eleutherascus were associated with plant health. Bacterial dysbiosis related to rhizome rot was due to the relative enrichment of Pectobacterium, Alcaligenes, Klebsiella, and Enterobacter. Similarly, an imbalance in the fungal community was caused by the enrichment of Gibellulopsis, Pyxidiophorales, and Plectosphaerella. Untargeted metabolomics analysis revealed several metabolites that drive microbiome assembly closely related to plant health in diverse microbial niches. At the same time, 6-({[3,4-dihydroxy-4-(hydroxymethyl)oxolan-2-yl]oxy}methyl)oxane-2,3,4,5-tetrol was present at the level of the entire healthy ginger plant. Lipids and lipid-like molecules were the most significant proportion of highly abundant metabolites associated with ginger plant health versus rhizome rot disease.ConclusionsOur research significantly improves our understanding of metabolome-driven microbiome structure to address crop protection impacts. The microbiome assembly rather than a particular microbe's occurrence drove ginger plant health. Most microbial species and metabolites have yet to be previously identified in ginger plants. The indigenous microbial communities and metabolites described can support future strategies to induce plant disease resistance. They provide a foundation for further exploring pathogens, biocontrol agents, and plant growth promoters associated with economically important crops. Video Abstract.

Project description:BackgroundMicroRNAs (miRNAs) are endogenous, non-coding small functional RNAs that govern the post-transcriptional regulatory system of gene expression and control the growth and development of plants. Ginger is an herb that is well-known for its flavor and medicinal properties. The genes involved in ginger rhizome development and secondary metabolism have been discovered, but the genome-wide identification of miRNAs and their overall expression profiles and targets during ginger rhizome development are largely unknown. In this study, we used BGISEQ-500 technology to perform genome-wide identification of miRNAs from the leaf, stem, root, flower, and rhizome of ginger during three development stages.ResultsIn total, 104 novel miRNAs and 160 conserved miRNAs in 28 miRNA families were identified. A total of 181 putative target genes for novel miRNAs and 2772 putative target genes for conserved miRNAs were predicted. Transcriptional factors were the most abundant target genes of miRNAs, and 17, 9, 8, 4, 13, 8, 3 conserved miRNAs and 5, 7, 4, 5, 5, 15, 9 novel miRNAs showed significant tissue-specific expression patterns in leaf, stem, root, flower, and rhizome. Additionally, 53 miRNAs were regarded as rhizome development-associated miRNAs, which mostly participate in metabolism, signal transduction, transport, and catabolism, suggesting that these miRNAs and their target genes play important roles in the rhizome development of ginger. Twelve candidate miRNA target genes were selected, and then, their credibility was confirmed using qRT-PCR. As the result of qRT-PCR analysis, the expression of 12 candidate target genes showed an opposite pattern after comparison with their miRNAs. The rhizome development system of ginger was observed to be governed by miR156, miR319, miR171a_2, miR164, and miR529, which modulated the expression of the SPL, MYB, GRF, SCL, and NAC genes, respectively.ConclusionThis is a deep genome-wide investigation of miRNA and identification of miRNAs involved in rhizome development in ginger. We identified 52 rhizome-related miRNAs and 392 target genes, and this provides an important basis for understanding the molecular mechanisms of the miRNA target genes that mediate rhizome development in ginger.

Dataset Information

Silica Nanoparticles Enhance the Disease Resistance of Ginger to Rhizome Rot during Postharvest Storage.

Publications

Silica Nanoparticles Enhance the Disease Resistance of Ginger to Rhizome Rot during Postharvest Storage.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets