Isolation of Potato Endophytes and Screening of Chaetomium globosum Antimicrobial Genes.

ABSTRACT: Antimicrobial peptides (AMPs) have natural antibacterial activities that pathogens find difficult to overcome. As a result of this occurrence, AMPs can act as an important substitute against the microbial resistance. In this study, we used plate confrontation tests to screen out 20 potential endophytes from potato tubers. Among them, endophyte F5 was found to significantly inhibit the growth of five different pathogenic fungi. Following that, phylogenetic analysis revealed that the internal transcribed spacer (ITS) sequences were 99% identical to Chaetomium globosum corresponding sequences. Thereafter, the Bacillus subtilis expression system was used to create a C. globosum cDNA library in order to isolate the resistance genes. Using this approach, the resistance gene

SUBMITTER: Zhang J 

PROVIDER: S-EPMC9099842 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature