Analysis of Myxococcus xanthus Vegetative Biofilms With Microtiter Plates.

ABSTRACT: The bacterium Myxococcus xanthus forms both developmental and vegetative types of biofilms. While the former has been studied on both agar plates and submerged surfaces, the latter has been investigated predominantly on agar surfaces as swarming colonies. Here we describe the development of a microplate-based assay for the submerged biofilms of M. xanthus under vegetative conditions. We examined the impacts of inoculation, aeration, and temperature to optimize the conditions for the assay. Aeration was observed to be critical for the effective development of submerged biofilms by M. xanthus, an obligate aerobic bacterium. In addition, temperature plays an important role in the development of M. xanthus submerged biofilms. It is well established that the formatio

SUBMITTER: Dye KJ 

PROVIDER: S-EPMC9100584 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature