Does Intracellular Metabolism Render Gemcitabine Uptake Undetectable in Mass Spectrometry?

ABSTRACT: The ergothioneine transporter ETT (formerly OCTN1; human gene symbol SLC22A4) is a powerful and highly specific transporter for the uptake of ergothioneine (ET). Recently, Sparreboom et al. reported that the ETT would transport nucleosides and nucleoside analogues such as cytarabine and gemcitabine with the highest efficiency. In our assay system, we could not detect any such transport. Subsequently, Sparreboom suggested that the intracellular metabolization of the nucleosides occurs so fast that the original compounds cannot be detected by LC-MS/MS after inward transport. Our current experiments with 293 cells disprove this hypothesis. Uptake of gemcitabine was easily detected by LC-MS/MS measurements when we expressed the Na⁺/nucleoside cotransporter CNT3 (SLC28A3

SUBMITTER: Muller JP 

PROVIDER: S-EPMC9101085 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature