TLR2/TLR4-Enhanced TIPE2 Expression Is Involved in Post-Hemorrhagic Shock Mesenteric Lymph-Induced Activation of CD4+T Cells.

ABSTRACT:

Purpose

Post hemorrhagic shock mesenteric lymph (PHSML) return contributes to CD4⁺ T cell dysfunction, which leads to immune dysfunction and uncontrolled inflammatory response. Tumor necrosis factor α induced protein 8 like-2 (TIPE2) is one of the essential proteins to maintain the immune homeostasis. This study investigated the role of TIPE2 in regulation of CD4⁺ T lymphocyte function in interaction of PHSML and TLR2/TLR4.

Methods

The splenic CD4⁺ T cells were isolated from various mice (WT, TLR2^-/-, TLR4^-/-) by immunomagnetic beads, and stimulated with PHSML, normal lymphatic fluid (NML), respectively. Application of TIPE2-carrying interfering fragments of lentivirus were transfected to WT, TLR4^-/-, and TLR2

SUBMITTER: Du HB 

PROVIDER: S-EPMC9101470 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature