Effects of bowel preparation on intestinal bacterial associated urine and faecal metabolites and the associated faecal microbiome.

ABSTRACT:

Background

Urinary and faecal metabolic profiling have been extensively studied in gastrointestinal diseases as potential diagnostic markers, and to enhance our understanding of the intestinal microbiome in the pathogenesis these conditions. The impact of bowel cleansing on the microbiome has been investigated in several studies, but limited to just one study on the faecal metabolome.

Aim

To compare the effects of bowel cleansing on the composition of the faecal microbiome, and the urine and faecal metabolome.

Methods

Urine and faecal samples were obtained from eleven patients undergoing colonoscopy at baseline, and then at day 3 and week 6 after colonoscopy. 16S rRNA gene sequencing was used to analyse changes in the microbiome, and metabonomic analysis was performed

SUBMITTER: Powles STR 

PROVIDER: S-EPMC9101932 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature