Glycogene Expression Profile of Human Limbal Epithelial Cells with Distinct Clonogenic Potential.

ABSTRACT: Glycans function as valuable markers of stem cells but also regulate the ability of these cells to self-renew and differentiate. Approximately 2% of the human genome encodes for proteins that are involved in the biosynthesis and recognition of glycans. In the present study, we evaluated the expression of a small subset of glycogenes in human limbal epithelial cells with distinct clonogenic potential. Individual clones were classified as abortive or clonogenic, based on the fraction of the terminal colonies produced; clones leading exclusively to terminal colonies were referred to as abortive while those with half or fewer terminal colonies were referred to as clonogenic. An analysis of glycogene expression in clonogenic cultures revealed a high content of transcripts regulating the galacto

SUBMITTER: Guindolet D 

PROVIDER: S-EPMC9102009 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature