Genetic and Molecular Characterization of the Immortalized Murine Hepatic Stellate Cell Line GRX.

ABSTRACT: The murine cell line GRX has been introduced as an experimental tool to study aspects of hepatic stellate cell biology. It was established from livers of C3H/HeN mice that were infected with cercariae of Schistosoma mansoni. Although these cells display a myofibroblast phenotype, they can accumulate intracellular lipids and acquire a fat-storing lipocyte phenotype when treated with retinol, insulin, and indomethacin. We have performed genetic characterization of GRX and established a multi-loci short tandem repeat (STR) signature for this cell line that includes 18 mouse STR markers. Karyotyping further revealed that this cell line has a complex genotype with various chromosomal aberrations. Transmission electron microscopy revealed that GRX cells produce large quantities of viral p

SUBMITTER: Schroder SK 

PROVIDER: S-EPMC9102025 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature