Ablation of Tmcc2 Gene Impairs Erythropoiesis in Mice.

ABSTRACT: (1) Background: Transcriptomic and proteomic studies provide a wealth of new genes potentially involved in red blood cell (RBC) maturation or implicated in the pathogenesis of anemias, necessitating validation of candidate genes in vivo; (2) Methods: We inactivated one such candidate, transmembrane and coiled-coil domain 2 (Tmcc2) in mice, and analyzed the erythropoietic phenotype by light microscopy, transmission electron microscopy (TEM), and flow cytometry of erythrocytes and erythroid precursors; (3) Results: Tmcc2^-/- pups presented pallor and reduced body weight due to the profound neonatal macrocytic anemia with numerous nucleated RBCs (nRBCs) and occasional multinucleated RBCs. Tmcc2^-/- nRBCs had cytoplasmic intrusions into the nucleus and

SUBMITTER: Kumari R 

PROVIDER: S-EPMC9102901 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature