Development of a quantitative methylation-specific droplet digital PCR assay for detecting Dickkopf-related protein 3.

ABSTRACT:

Objective

The detection and monitoring of DNA methylation status in circulating tumor cell DNA (ctDNA) provides critical insights into cancer diagnosis and progression. The methylation status of the Dickkopf-related protein 3 (DKK3) promoter region is correlated with the metastasis and recurrence of multiple cancers. Thus, detecting the methylation status via non-invasive methods is essential for the diagnosis and prognosis of cancers. Using a droplet digital polymerase chain reaction approach, we have developed a highly sensitive and quantitative measurement of methylated and unmethylated DKK3 derived from circulating cell-free DNA (ccfDNA).

Results

We confirmed the specificity of droplet digital methylation specific polymerase chain reaction (ddMSP). We selected the optima

SUBMITTER: Araki K 

PROVIDER: S-EPMC9103039 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature