A Reliable Approach for Revealing Molecular Targets in Secondary Ion Mass Spectrometry.

ABSTRACT: Nano secondary ion mass spectrometry (nanoSIMS) imaging is a rapidly growing field in biological sciences, which enables investigators to describe the chemical composition of cells and tissues with high resolution. One of the major challenges of nanoSIMS is to identify specific molecules or organelles, as these are not immediately recognizable in nanoSIMS and need to be revealed by SIMS-compatible probes. Few laboratories have generated such probes, and none are commercially available. To address this, we performed a systematic study of probes initially developed for electron microscopy. Relying on nanoscale SIMS, we found that antibodies coupled to 6 nm gold particles are surprisingly efficient in terms of labeling specificity while offering a reliable detection threshold. These tools ena

SUBMITTER: Li F 

PROVIDER: S-EPMC9103194 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature