RNAi-Mediated Knockdown of Calreticulin3a Impairs Pollen Tube Growth in Petunia.

ABSTRACT: Pollen tube growth depends on several complex processes, including exo/endocytosis, cell wall biogenesis, intracellular transport, and cell signaling. Our previous results provided evidence that calreticulin (CRT)-a prominent calcium (Ca²⁺)-buffering molecular chaperone in the endoplasmic reticulum (ER) lumen-is involved in pollen tube formation and function. We previously cloned and characterized the CRT gene belonging to the CRT1/2 subgroup from Petunia hybrida (PhCRT1/2), and found that post-transcriptional silencing of PhCRT1/2 expression strongly impaired pollen tube growth in vitro. Here, we report cloning of a new PhCRT3a homolog; we identified the full-length cDNA sequence and described its molecular characteristics and phylogene

SUBMITTER: Wasag P 

PROVIDER: S-EPMC9103332 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature