Advanced Immune Cell Profiling by Multiparameter Flow Cytometry in Humanized Patient-Derived Tumor Mice.

ABSTRACT: "Humanized" mice have been widely used for the characterization of human cancer progression and as a powerful preclinical model. Standardization of multicolor phenotyping could help to identify immune cell patterns involved in checkpoint-related complications. Therefore, we applied established protocols for immune cell profiling to our humanized Patient-Derived Xenograft (hPDX) model. hPDX are characterized by the co-existence of a human immune system and a patient-derived tumor transplant. These mice possess a human-like immune system after CD34⁺ stem cell transplantation while the reconstitution level of the immune system was not related to the quantity of transplanted CD34⁺ cells. Contamination ≤ 1.2% by CD3⁺ cells in the hematopoietic stem cell (HSC) tr

SUBMITTER: Bruss C 

PROVIDER: S-EPMC9103756 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature