Comparison of Whole Blood Cryopreservation Methods for Extensive Flow Cytometry Immunophenotyping.

ABSTRACT: Fresh blood immunophenotyping by flow cytometry, based on the reliable simultaneous detection of several markers in a cell, is the method of choice to study the circulating human immune system. Especially in large and multicenter studies, high sample quality is difficult to achieve, and adequate collection and storage of samples with fine-tuned whole blood cryopreservation is mandatory. Here, we compared the quality of immunophenotypic data obtained from fresh blood with those obtained after five cryopreservation methods by quantifying the levels of 41 immune cell populations. They comprised B and T lymphocyte subsets and their maturation stages, as well as monocytes and granulocytes. Three methods used fixative solutions and two other methods used dimethyl sulfoxide solutions to preserve

SUBMITTER: Serra V 

PROVIDER: S-EPMC9103885 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature