Metal-Induced Fluorescence Quenching of Photoconvertible Fluorescent Protein DendFP.

ABSTRACT: Sensitive and accurate detection of specific metal ions is important for sensor development and can advance analytical science and support environmental and human medical examinations. Fluorescent proteins (FPs) can be quenched by specific metal ions and spectroscopically show a unique fluorescence-quenching sensitivity, suggesting their potential application as FP-based metal biosensors. Since the characteristics of the fluorescence quenching are difficult to predict, spectroscopic analysis of new FPs is important for the development of FP-based biosensors. Here we reported the spectroscopic and structural analysis of metal-induced fluorescence quenching of the photoconvertible fluorescent protein DendFP. The spectroscopic analysis showed that Fe²⁺, Fe³⁺, and Cu

SUBMITTER: Kim IJ 

PROVIDER: S-EPMC9104182 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature