Pls1 Is a Peroxisomal Matrix Protein with a Role in Regulating Lysine Biosynthesis.

ABSTRACT: Peroxisomes host essential metabolic enzymes and are crucial for human health and survival. Although peroxisomes were first described over 60 years ago, their entire proteome has not yet been identified. As a basis for understanding the variety of peroxisomal functions, we used a high-throughput screen to discover peroxisomal proteins in yeast. To visualize low abundance proteins, we utilized a collection of strains containing a peroxisomal marker in which each protein is expressed from the constitutive and strong TEF2 promoter. Using this approach, we uncovered 18 proteins that were not observed in peroxisomes before and could show their metabolic and targeting factor dependence for peroxisomal localization. We focus on one newly identified and uncharacterized matrix protein, Ynl09

SUBMITTER: David Y 

PROVIDER: S-EPMC9104712 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature