Structure Elucidation of Fucan Sulfate from Sea Cucumber Holothuria fuscopunctata through a Bottom-Up Strategy and the Antioxidant Activity Analysis.

ABSTRACT: Fucan sulfate I (FSI) from the sea cucumber Holothuria fuscopunctata was purified and its structure was clarified based on a bottom-up strategy. The unambiguous structures of a series of oligosaccharides including disaccharides, trisaccharides, and tetrasaccharides, which were released from mild acid hydrolysis of FSI, were identified by one-dimensional (1D)/two-dimensional (2D) nuclear magnetic resonance (NMR) and mass spectrometry (MS) analysis. All the glycosidic bonds in these oligosaccharides were presented as α1,3 linkages confirmed by correlated signals from their ¹H-¹H ROESY and ¹H-¹³C HMBC spectra. The structural sequence of these oligosaccharides formed by Fuc_2S4S, Fuc_2S, and non-sulfated ones (Fuc_0S

SUBMITTER: Gao L 

PROVIDER: S-EPMC9105098 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature