The Nitrogen Atom of Vitamin B₆ Is Essential for the Catalysis of Radical Aminomutases.

ABSTRACT: Radical aminomutases are pyridoxal 5'-phosphate (PLP, a B₆ vitamer)-dependent enzymes that require the generation of a 5'-deoxyadenosyl radical to initiate the catalytic cycle, to perform a 1,2 amino group shift reaction. The role of the nitrogen atom of PLP in radical aminomutases has not been investigated extensively yet. We report an alternative synthetic procedure to provide easy access to 1-deazaPLP (dAPLP), an isosteric analog of PLP which acts as a probe for studying the role of the nitrogen atom. Our results revealed that lysine 5,6-aminomutase (5,6-LAM), a radical aminomutase, reconstituted with dAPLP cannot turn over a substrate, demonstrating that the nitrogen atom is essential for radical aminomutases. In contrast, biochemical and spectroscopic studies on the S238A v

SUBMITTER: Maity AN 

PROVIDER: S-EPMC9105233 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature