High-throughput characterization of the role of non-B DNA motifs on promoter function.

ABSTRACT: lternative DNA conformations, termed non-B DNA structures, can affect transcription, but the underlying mechanisms and their functional impact have not been systematically characterized. Here, we used computational genomic analyses coupled with massively parallel reporter assays (MPRAs) to show that certain non-B DNA structures have a substantial effect on gene expression. Genomic analyses found that non-B DNA structures at promoters harbor an excess of germline variants. Analysis of multiple MPRAs, including a promoter library specifically designed to perturb non-B DNA structures, functionally validated that Z-DNA can significantly affect promoter activity. We also observed that biophysical properties of non-B DNA motifs, such as the length of Z-DNA motifs and the orientation of G-quadrup

SUBMITTER: Georgakopoulos-Soares I 

PROVIDER: S-EPMC9105345 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature