Compound-Specific ¹⁴N/¹⁵N Analysis of Amino Acid Trimethylsilylated Derivatives from Plant Seed Proteins.

ABSTRACT: Isotopic analyses of plant samples are now of considerable importance for food certification and plant physiology. In fact, the natural nitrogen isotope composition (δ¹⁵N) is extremely useful to examine metabolic pathways of N nutrition involving isotope fractionations. However, δ¹⁵N analysis of amino acids is not straightforward and involves specific derivatization procedures to yield volatile derivatives that can be analysed by gas chromatography coupled to isotope ratio mass spectrometry (GC-C-IRMS). Derivatizations other than trimethylsilylation are commonly used since they are believed to be more reliable and accurate. Their major drawback is that they are not associated with metabolite databases allowing identification of derivatives and by-products. Here, we re

SUBMITTER: Domergue JB 

PROVIDER: S-EPMC9105707 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature