Dataset Information

Tiller Angle Control 1 Is Essential for the Dynamic Changes in Plant Architecture in Rice.

ABSTRACT: Plant architecture is dynamic as plants develop. Although many genes associated with specific plant architecture components have been identified in rice, genes related to underlying dynamic changes in plant architecture remain largely unknown. Here, we identified two highly similar recombinant inbred lines (RILs) with different plant architecture: RIL-Dynamic (D) and RIL-Compact (C). The dynamic plant architecture of RIL-D is characterized by 'loose^{tiller angle} (tillering stage)-compact (heading stage)-loose^{curved stem} (maturing stage)' under natural long-day (NLD) conditions, and 'loose^{tiller angle} (tillering and heading stages)-loose^{tiller angle and curved stem} (maturing stage)' under natural short-day (NSD) conditions, while RIL-C exhibits a compact plant architecture both under NLD and NSD conditions throughout growth. The candidate locus was mapped to the chromosome 9 tail via the rice 8K chip assay and map-based cloning. Sequencing, complementary tests, and gene knockout tests demonstrated that Tiller Angle Control 1 (TAC1) is responsible for dynamic plant architecture in RIL-D. Moreover, TAC1 positively regulates loose plant architecture, and high TAC1 expression cannot influence the expression of tested tiller-angle-related genes. Our results reveal that TAC1 is necessary for the dynamic changes in plant architecture, which can guide improvements in plant architecture during the modern super rice breeding.

SUBMITTER: Wang H

PROVIDER: S-EPMC9105778 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature

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<i>Tiller Angle Control 1</i> Is Essential for the Dynamic Changes in Plant Architecture in Rice.

Wang Hong H Tu Ranran R Sun Lianping L Wang Dongfei D Ruan Zheyan Z Zhang Yue Y Peng Zequn Z Zhou Xingpeng X Fu Junlin J Liu Qunen Q Wu Weixun W Zhan Xiaodeng X Shen Xihong X Zhang Yingxin Y Cao Liyong L Cheng Shihua S

International journal of molecular sciences 20220430 9

Plant architecture is dynamic as plants develop. Although many genes associated with specific plant architecture components have been identified in rice, genes related to underlying dynamic changes in plant architecture remain largely unknown. Here, we identified two highly similar recombinant inbred lines (RILs) with different plant architecture: RIL-Dynamic (D) and RIL-Compact (C). The dynamic plant architecture of RIL-D is characterized by 'loose<sup>tiller angle</sup> (tillering stage)-compa ...[more]

PMID: 35563391

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Project description:IntroductionPlants can adapt their growth to optimize light capture in competitive environments, with branch angle being a crucial factor influencing plant phenotype and physiology. Decreased branch angles in cereal crops have been shown to enhance productivity in high-density plantings. The Tiller Angle Control (TAC1) gene, known for regulating tiller inclination in rice and corn, has been found to control branch angle in eudicots. Manipulating TAC1 in field crops like cotton offers the potential for improving crop productivity.MethodsUsing a homolog-based methodology, we examined the distribution of TAC1-related genes in cotton compared to other angiosperms. Furthermore, tissue-specific qPCR analysis unveiled distinct expression patterns of TAC1 genes in various cotton tissues. To silence highly expressed specific TAC1 homeologs in the stem, we applied CRISPR-Cas9 gene editing and Agrobacterium-mediated transformation, followed by genotyping and subsequent phenotypic validation of the mutants.ResultsGene duplication events of TAC1 specific to the Gossypium lineage were identified, with 3 copies in diploid progenitors and 6 copies in allotetraploid cottons. Sequence analysis of the TAC1 homeologs in Gossypium hirsutum revealed divergence from other angiosperms with 1-2 copies, suggesting possible neo- or sub-functionalization for the duplicated copies. These TAC1 homeologs exhibited distinct gene expression patterns in various tissues over developmental time, with elevated expression of A11G109300 and D11G112200, specifically in flowers and stems, respectively. CRISPR-mediated loss of these TAC1 homeologous genes resulted in a reduction in branch angle and altered petiole angles, and a 5 to 10-fold reduction in TAC1 expression in the mutants, confirming their role in controlling branch and petiole angles. This research provides a promising strategy for genetically engineering branch and petiole angles in commercial cotton varieties, potentially leading to increased productivity.

Dataset Information

Tiller Angle Control 1 Is Essential for the Dynamic Changes in Plant Architecture in Rice.

Publications

<i>Tiller Angle Control 1</i> Is Essential for the Dynamic Changes in Plant Architecture in Rice.

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