Dealing with inconclusive SARS-CoV-2 PCR samples-Our experience.

ABSTRACT:

Purpose

Early confirmation of SARS-CoV-2 is a key point in the timely management of infected patients and contact persons. Routine diagnostics of COVID-19 cases relies on RT-PCR detection of two or three unique sequences of the virus. A serious problem for the laboratories is how to interpret inconclusive samples which are positive for only one of the SARS-CoV-2 specific genes.

Materials and methods

A total of 16364 naso-oropharyngeal swabs were collected and tested with SARS-CoV-2 Real-TM kit (Sacace Biotechnologies, Italy) between May and September 2020. We retrospectively analyzed their amplification plots to determine the number of inconclusive samples. We also reviewed the medical records to summarize the patient's COVID-19 testing history and basic demographic characte

SUBMITTER: Stoykova Z 

PROVIDER: S-EPMC9106147 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature