Highly multiplexed immune repertoire sequencing links multiple lymphocyte classes with severity of response to COVID-19.

ABSTRACT:

Background

Disease progression of subjects with coronavirus disease 2019 (COVID-19) varies dramatically. Understanding the various types of immune response to SARS-CoV-2 is critical for better clinical management of coronavirus outbreaks and to potentially improve future therapies. Disease dynamics can be characterized by deciphering the adaptive immune response.

Methods

In this cross-sectional study we analyzed 117 peripheral blood immune repertoires from healthy controls and subjects with mild to severe COVID-19 disease to elucidate the interplay between B and T cells. We used an immune repertoire Primer Extension Target Enrichment method (immunoPETE) to sequence simultaneously human leukocyte antigen (HLA) restricted T cell receptor beta chain (TRB) and unrestricted T cel

SUBMITTER: Dannebaum R 

PROVIDER: S-EPMC9106482 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature