Ontology highlight
ABSTRACT:
SUBMITTER: Krucinska J
PROVIDER: S-EPMC9106665 | biostudies-literature | 2022 May
REPOSITORIES: biostudies-literature
Krucinska Jolanta J Lombardo Michael N MN Erlandsen Heidi H Estrada Alexavier A Si Debjani D Viswanathan Kishore K Wright Dennis L DL
Communications biology 20220513 1
Two plasmid-encoded dihydrofolate reductase (DHFR) isoforms, DfrA1 and DfrA5, that give rise to high levels of resistance in Gram-negative bacteria were structurally and biochemically characterized to reveal the mechanism of TMP resistance and to support phylogenic groupings for drug development against antibiotic resistant pathogens. Preliminary screening of novel antifolates revealed related chemotypes that showed high levels of inhibitory potency against Escherichia coli chromosomal DHFR (EcD ...[more]