CDC7-independent G1/S transition revealed by targeted protein degradation.

ABSTRACT: The entry of mammalian cells into the DNA synthesis phase (S phase) represents a key event in cell division¹. According to current models of the cell cycle, the kinase CDC7 constitutes an essential and rate-limiting trigger of DNA replication, acting together with the cyclin-dependent kinase CDK2. Here we show that CDC7 is dispensable for cell division of many different cell types, as determined using chemical genetic systems that enable acute shutdown of CDC7 in cultured cells and in live mice. We demonstrate that another cell cycle kinase, CDK1, is also active during G1/S transition both in cycling cells and in cells exiting quiescence. We show that CDC7 and CDK1 perform functionally redundant roles during G1/S transition, and at least one of these kinases must be present to a

SUBMITTER: Suski JM 

PROVIDER: S-EPMC9106935 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature