Derivation of human triploid trophoblast stem cells.

ABSTRACT:

Purpose

Human trophoblast stem cells (hTSCs) are counterparts of the precursor cells of the placenta and are valuable cell models for the study of placental development and the pathogenesis of placental diseases. The aim of this work was to establish a triploid human TSC (hTSC^3PN) derived from the tripronuclear embryos, which are clinically discarded but readily available, for potential applications in basic placental research and disease modeling.

Methods

Eighteen tripronuclear human zygotes from IVF were collected and cultured for 5-6 days. Five high-quality blastocysts were harvested and were individually cultured in hTSC medium. Finally, two hTSC lines were established after 10 days and could be passaged stably.

Results

The karyotyping analysis showed t

SUBMITTER: Kong X 

PROVIDER: S-EPMC9107551 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature