Identification and genome characterization of novel parechovirus sequences from Hipposideros armiger in China.

ABSTRACT:

Background

Bats were identified as a natural reservoir of emerging and re-emerging infectious pathogens threatening human health and life.

Methods

This study collected 21 fecal samples of Hipposideros armiger in Mengla County of Xishuangbanna Prefecture Yunnan Province to combine one pool for viral metagenomic sequencing.

Results

Two nearly complete genomes of parechoviruses, BPeV11 and BPeV20, were sequenced. Genome analysis revealed that BPeV11 and BPeV20 follow a 3-3-4 genome layout: 5' UTR-VP0-VP3-VP1-2A-2B-2C-3A-3B-3C-3D-3' UTR. The prevalence of BPev11 and BPev20 by Nested-PCR showed that 1 of 21 fecal samples was positive. Based on amino acid identity comparison and phylogenetic analysis of P1, 2C, and 3D, BPeV11 and BPeV20 were closely related to but distinct

SUBMITTER: Zeng J 

PROVIDER: S-EPMC9107582 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature