Metabolic engineering of Clostridium ljungdahlii for the production of hexanol and butanol from CO₂ and H₂.

ABSTRACT:

Background

The replacement of fossil fuels and petrochemicals with sustainable alternatives is necessary to mitigate the effects of climate change and also to counteract diminishing fossil resources. Acetogenic microorganisms such as Clostridium spp. are promising sources of fuels and basic chemical precursors because they efficiently utilize CO and CO₂ as carbon source. However the conversion into high titers of butanol and hexanol is challenging.

Results

Using a metabolic engineering approach we transferred a 17.9-kb gene cluster via conjugation, containing 13 genes from C. kluyveri and C. acetobutylicum for butanol and hexanol biosynthesis, into C. ljungdahlii. Plasmid-based expression resulted in 1075 mg L^-1 butanol and 133 mg L^-1 hexanol

SUBMITTER: Lauer I 

PROVIDER: S-EPMC9107641 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature