RNA adenosine modifications related to prognosis and immune infiltration in osteosarcoma.

ABSTRACT:

Background

RNA adenosine modifications, which are primarily mediated by "writer" enzymes (RMWs), play a key role in epigenetic regulation in various biological processes, including tumorigenesis. However, the expression and prognostic role of these genes in osteosarcoma (OS) remain unclear.

Methods

Univariate and multivariate Cox analyses were used to construct the RMW signature for OS using Target datasets. RMW expression in OS tissue was detected by qPCR analysis. Xcell and GSVA were used to determine the relationship between RMWs and immune infiltration. The DGIdb and CMap databases were used for drug prediction. In vivo and in vitro experiments showed that strophanthidin elicited antitumor activity against OS.

Results

A 3-RMW (CSTF2, ADAR and WTAP) prognostic sign

SUBMITTER: Chen S 

PROVIDER: S-EPMC9107650 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature