Microfluidic screening and genomic mutation identification for enhancing cellulase production in Pichia pastoris.

ABSTRACT:

Background

Pichia pastoris is a widely used host organism for heterologous production of industrial proteins, such as cellulases. Although great progress has been achieved in improving protein expression in P. pastoris, the potential of the P. pastoris expression system has not been fully explored due to unknown genomic impact factors. Recently, whole-cell directed evolution, employing iterative rounds of genome-wide diversity generation and high-throughput screening (HTS), has been considered to be a promising strategy in strain improvement at the genome level.

Results

In this study, whole-cell directed evolution of P. pastoris, employing atmospheric and room temperature plasma (ARTP) mutagenesis and droplet-based microfluidic HTS, was developed to improve heterogenous cell

SUBMITTER: Yuan H 

PROVIDER: S-EPMC9107654 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature