Biosynthesis of eriodictyol from tyrosine by Corynebacterium glutamicum.

ABSTRACT:

Background

Eriodictyol is a bioactive flavonoid compound that shows potential applications in medicine development and food processing. Microbial synthesis of eriodictyol has been attracting increasing attention due to several benefits. In this study, we employed a GRAS strain Corynebacterium glutamicum as the host to produce eriodictyol directly from tyrosine.

Results

We firstly optimized the biosynthetic module of naringenin, the upstream intermediate for eriodictyol production, through screening of different gene orthologues. Next, to improve the level of the precursor malonyl-CoA necessary for naringenin production, we introduced matB and matC from Rhizobium trifolii into C. glutamicum to convert extracellular malonate to intracellular malonyl-CoA. This combinatorial eng

SUBMITTER: Wu X 

PROVIDER: S-EPMC9107716 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature