Elevated BTG2 improves the radiosensitivity of non-small cell lung cancer (NSCLC) through apoptosis.

ABSTRACT:

Background

To identify radio-responsive genes and explore the biological function of encoded proteins in non-small cell lung cancer (NSCLC).

Methods

Radio-responsive genes in irradiated H460 cells were screened from microarray data deposited in the Gene Expression Omnibus (GEO) database. A quantitative real time polymerase chain reaction assay was used to detect the expression of candidate radio-responsive genes in irradiated cells. CCK-8 assay, EDU assay, clone formation assay, immunofluorescence and flow cytometry were conducted to evaluate the biological function of B cell translocation gene 2 (BTG2) in NSCLC.

Results

Bioinformatic analysis using GES20549 showed that BTG2 was a radio-responsive gene in irradiated H460 cells. The mRNA expression level of BTG2 was lo

SUBMITTER: Zhu C 

PROVIDER: S-EPMC9108063 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature