Development of a Polymerase Spiral Reaction-Based Isothermal Assay for Rapid Identification of Thrips palmi.

ABSTRACT: Thrips cause considerable economic losses to a wide range of food, feed, and forest crops. They also transmit several plant viruses. Being cryptic, it is often difficult to distinguish thrips species in crops and large consignments by conventional methods. Melon thrips (Thrips palmi Karny, Thysanoptera: Thripidae) is an invasive insect pest of vegetables, legumes, and ornamentals besides being vector to several viruses. It poses a threat to domestic and international plant biosecurity and can invade and establish in new areas. Here, we report a polymerase spiral reaction (PSR)-based isothermal assay for rapid, sensitive, specific, low-cost, and on-site detection of T. palmi. To the best of our knowledge, this is the first application of PSR in the identification of any insect

SUBMITTER: Jangra S 

PROVIDER: S-EPMC9108268 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature