Bizonal cardiac engineered tissues with differential maturation features in a mid-throughput multimodal bioreactor.

ABSTRACT: Functional three-dimensional (3D) engineered cardiac tissue (ECT) models are essential for effective drug screening and biological studies. Application of physiological cues mimicking those typical of the native myocardium is known to promote the cardiac maturation and functionality in vitro. Commercially available bioreactors can apply one physical force type at a time and often in a restricted loading range. To overcome these limitations, a millimetric-scale microscope-integrated bioreactor was developed to deliver multiple biophysical stimuli to ECTs. In this study, we showed that the single application of auxotonic loading (passive) generated a bizonal ECT with a unique cardiac maturation pattern. Throughout the statically cultured constructs and in the ECT region exposed to hig

SUBMITTER: Pisanu A 

PROVIDER: S-EPMC9108516 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature