Injection of human neuroblastoma cells into neural crest streams in live zebrafish embryos.

ABSTRACT: Cancer cell behavior is highly microenvironment dependent, but we have a limited understanding of malignant cell-microenvironment interactions in vivo. Here, we describe a protocol for xenotransplanting human neuroblastoma (NB) cells into streams of migrating neural crest stem cells in zebrafish embryos, followed by confocal time-lapse imaging and cell tracking. This high-resolution model system facilitates the quantitative spatiotemporal analysis of cancer cell-cell and cell-environment interactions. For complete details on the use and execution of this protocol, please refer to Treffy et al. (2021).

SUBMITTER: Ibarra BA 

PROVIDER: S-EPMC9108679 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature