Two Liberibacter Proteins Combine to Suppress Critical Innate Immune Defenses in Citrus.

ABSTRACT: We adopted a systems-based approach to determine the role of two Candidatus Liberibacter asiaticus (CLas) proteins, LasP ₂₃₅ and Effector 3, in Huanglongbing (HLB) pathogenesis. While a published work suggests the involvement of these CLas proteins HLB pathogenesis, the exact structure-based mechanism of their action has not been elucidated. We conducted the following experiments to determine the structure-based mechanisms of action. First, we immunoprecipitated the interacting citrus protein partners of LasP ₂₃₅ and Effector 3 from the healthy and CLas-infected Hamlin extracts and identified them by Liquid Chromatography with tandem mass spectrometry (LC-MS/MS). Second, we performed a split green fluorescent protein (G

SUBMITTER: Basu S 

PROVIDER: S-EPMC9108871 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature