Large-scale engineering of hiPSC-derived nephron sheets and cryopreservation of their progenitors.

ABSTRACT:

Background

The generation of human induced pluripotent stem cells (hiPSCs) has opened a world of opportunities for stem cell-based therapies in regenerative medicine. Currently, several human kidney organoid protocols are available that generate organoids containing kidney structures. However, these kidney organoids are relatively small ranging up to 0.13 cm² and therefore contain a small number of nephrons compared to an adult kidney, thus defying the exploration of future use for therapy.

Method

We have developed a scalable, easily accessible, and reproducible protocol to increase the size of the organoid up to a nephron sheet of 2.5 cm² up to a maximum of 12.6 cm² containing a magnitude of nephrons.

Results

Confocal microscopy showe

SUBMITTER: Wiersma LE 

PROVIDER: S-EPMC9109372 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature