Immortalization effect of SV40T lentiviral vectors on canine corneal epithelial cells.

ABSTRACT:

Background

Primary canine corneal epithelial cells (CCECs) easily become senescent, and cell proliferation is limited. Therefore, sampling for experimentation requires a large number of animals, which is problematic in terms of animal welfare and fails to maintain the stability of the cells for in vitro analyses.

Results

In this study, CCECs were separated and purified by trypsin and dispase II enzymatic analysis. Next, the cells were immortalized by transfection with a lentiviral vector expressing Simian vacuolating virus 40 large T (SV40T). The immortalized canine corneal epithelial cell line (CCEC-SV40T) was established by serial passages and monoclonal selection. The biological characteristics of CCEC-SV40T cells were evaluated based on the cell proliferation rate, cell

SUBMITTER: Guo L 

PROVIDER: S-EPMC9109393 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature