Characterization of vitellogenin concentration in male fathead minnow mucus compared to plasma, and liver mRNA.

ABSTRACT: The objective of this study was to characterize vitellogenin (VTG) protein in male fathead minnow (Pimephales promelas) mucus compared with more conventional measures in plasma and mRNA isolated from liver. To assess the intensity and duration of changes in mucus VTG concentrations, male fathead minnows were exposed to 17α-ethinylestradiol (EE2) for 7 days with a subsequent depuration period of 14 days. The experiment was conducted in a flow-through system to maintain a consistent concentration of EE2 at a nominal EC₅₀ concentration of 2.5 ng/L and high concentration of 10 ng/L as a positive control. Mucus, plasma and liver were sampled at regular intervals throughout the study. Relative abundance of vtg mRNA increased after 2 days of exposure and returned to control levels afte

SUBMITTER: See MJ 

PROVIDER: S-EPMC9109421 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature