Development of a new antigen-based microarray platform for screening and detection of human IgG antibodies against SARS-CoV-2.

ABSTRACT: Strategies to contain the current SARS-CoV-2 pandemic rely, beside vaccinations, also on molecular and serological testing. For any kind of assay development, screening for the optimal antigen is essential. Here we describe the verification of a new protein microarray with different commercially available preparations significant antigens of SARS-CoV-2 that can be used for the evaluation of the performance of these antigens in serological assays and for antibody screening in serum samples. Antigens of other pathogens that are addressed by widely used vaccinations were also included. To evaluate the accuracy of 21 different antigens or antigen preparations on the microarray, receiver operating characteristics (ROC) curve analysis using ELISA results as reference were performed. Except for a

SUBMITTER: Burgold-Voigt S 

PROVIDER: S-EPMC9109672 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature