Immunogenicity of full-length P. vivax rPvs48/45 protein formulations in BALB/c mice.

ABSTRACT:

Background

Pvs48/45 is a Plasmodium vivax gametocyte surface protein involved in the parasite fertilization process. Previous studies showed that Pvs48/45 proteins expressed in Escherichia coli (E. coli) and Chinese hamster ovary (CHO) cells were highly immunoreactive with sera from malaria-endemic areas and highly immunogenic in animal models. Here the immunogenicity in mice of three different vaccine formulations was compared.

Methods

Recombinant (r) Pvs48/45 proteins were expressed in E. coli and CHO, purified, formulated in Alhydrogel, GLA-SE and Montanide ISA-51 adjuvants and used to immunize BALB/c mice. Animals were immunized on days 0, 20 and 40, and serum samples were collected for serological analyses of specific antibody responses using ELISA and immunofluorescenc

SUBMITTER: Arevalo-Herrera M 

PROVIDER: S-EPMC9109962 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature