Sensitive identification of neoantigens and cognate TCRs in human solid tumors.
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ABSTRACT:
SUBMITTER: Arnaud M
PROVIDER: S-EPMC9110298 | biostudies-literature | 2022 May
REPOSITORIES: biostudies-literature
Publications
Sensitive identification of neoantigens and cognate TCRs in human solid tumors.
Arnaud Marion M Chiffelle Johanna J Genolet Raphael R Navarro Rodrigo Blanca B Perez Marta A S MAS Huber Florian F Magnin Morgane M Nguyen-Ngoc Tu T Guillaume Philippe P Baumgaertner Petra P Chong Chloe C Stevenson Brian J BJ Gfeller David D Irving Melita M Speiser Daniel E DE Schmidt Julien J Zoete Vincent V Kandalaft Lana E LE Bassani-Sternberg Michal M Bobisse Sara S Coukos George G Harari Alexandre A
Nature biotechnology 20211115 5
The identification of patient-specific tumor antigens is complicated by the low frequency of T cells specific for each tumor antigen. Here we describe NeoScreen, a method that enables the sensitive identification of rare tumor (neo)antigens and of cognate T cell receptors (TCRs) expressed by tumor-infiltrating lymphocytes. T cells transduced with tumor antigen-specific TCRs identified by NeoScreen mediate regression of established tumors in patient-derived xenograft mice. ...[more]
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