Dataset Information

Purification, identification and characterization of Nag2 N-acetylglucosaminidase from Trichoderma virens strain mango.

ABSTRACT:

Background

N-acetylglucosaminidase (NAGase) could liberate N-acetylglucosamine (GlcNAc) from GlcNAc-containing oligosaccharides. Trichoderma spp. is an important source of chitinase, particularly NAGase for industrial use. nag1 and nag2 genes encoding NAGase, are found in the genome in Trichoderma spp. The deduced Nag1 and Nag2 shares ~ 55% homology in Trichoderma virens. Most studies were focus on Nag1 and nag1 previously.

Results

The native NAGase (TvmNAG2) was purified to homogeneity with molecular mass of ~ 68 kDa on SDS-PAGE analysis, and identified as Nag2 by MALDI/MS analysis from an isolate T. virens strain mango. RT-PCR analyses revealed that only nag2 gene was expressed in liquid culture of T. virens, while both of nag1 and nag2 were expressed in T. virens cultured on the plates. TvmNAG2 was thermally stable up to 60 °C for 2 h, and the optimal pH and temperature were 5.0 and 60-65 °C, respectively, using p-nitrophenyl-N-acetyl-β-D-glucosaminide (pNP-NAG) as substrate. The hydrolytic product of colloidal chitin by TvmNAG2 was suggested to be GlcNAc based on TLC analyses. Moreover, TvmNAG2 possesses antifungal activity, inhibiting the mycelium growth of Sclerotium rolfsii. And it was resistant to the proteolysis by papain and trypsin.

Conclusions

The native Nag2, TvmNAG2 was purified and identified from T. virens strain mango, as well as enzymatic properties. To our knowledge, it is the first report with the properties of native Trichoderma Nag2.

SUBMITTER: Huang JH

PROVIDER: S-EPMC9110600 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature

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Publications

Purification, identification and characterization of Nag2 N-acetylglucosaminidase from Trichoderma virens strain mango.

Huang Jheng-Hua JH Zeng Feng-Jin FJ Guo Jhe-Fu JF Huang Jian-Yuan JY Lin Hua-Chian HC Lo Chaur-Tsuen CT Chou Wing-Ming WM

Botanical studies 20220517 1

<h4>Background</h4>N-acetylglucosaminidase (NAGase) could liberate N-acetylglucosamine (GlcNAc) from GlcNAc-containing oligosaccharides. Trichoderma spp. is an important source of chitinase, particularly NAGase for industrial use. nag1 and nag2 genes encoding NAGase, are found in the genome in Trichoderma spp. The deduced Nag1 and Nag2 shares ~ 55% homology in Trichoderma virens. Most studies were focus on Nag1 and nag1 previously.<h4>Results</h4>The native NAGase (TvmNAG2) was purified to homog ...[more]

PMID: 35578140

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Dataset Information

Purification, identification and characterization of Nag2 N-acetylglucosaminidase from Trichoderma virens strain mango.

Background

Results

Conclusions

Publications

Purification, identification and characterization of Nag2 N-acetylglucosaminidase from Trichoderma virens strain mango.

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