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Novel electron microscopic staining method using traditional dye, hematoxylin.

ABSTRACT: Uranyl acetate (UA) has been routinely used as a staining solution for ultrathin sections used in biological electron microscopy. As a radioactive nuclear material, UA is subject to strict international regulations. To develop an alternative and easy-to-use staining method for ultrathin sections, we examined various commercial light microscopic dyes. We found that Mayer's hematoxylin followed by Reynold's lead citrate solution showed staining results comparable to UA and Reynold's lead citrate solution, and this method is therefore suggested as a reliable and promising alternative to UA staining.

SUBMITTER: Sasaki H

PROVIDER: S-EPMC9110702 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature

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Novel electron microscopic staining method using traditional dye, hematoxylin.

Sasaki Hiroyuki H Arai Hisako H Kikuchi Emi E Saito Hideki H Seki Keiko K Matsui Takeshi T

Scientific reports 20220516 1

Uranyl acetate (UA) has been routinely used as a staining solution for ultrathin sections used in biological electron microscopy. As a radioactive nuclear material, UA is subject to strict international regulations. To develop an alternative and easy-to-use staining method for ultrathin sections, we examined various commercial light microscopic dyes. We found that Mayer's hematoxylin followed by Reynold's lead citrate solution showed staining results comparable to UA and Reynold's lead citrate s ...[more]

PMID: 35577902

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