Disordered-Ordered Protein Binary Classification by Circular Dichroism Spectroscopy.

ABSTRACT: Intrinsically disordered proteins lack a stable tertiary structure and form dynamic conformational ensembles due to their characteristic physicochemical properties and amino acid composition. They are abundant in nature and responsible for a large variety of cellular functions. While numerous bioinformatics tools have been developed for in silico disorder prediction in the last decades, there is a need for experimental methods to verify the disordered state. CD spectroscopy is widely used for protein secondary structure analysis. It is usable in a wide concentration range under various buffer conditions. Even without providing high-resolution information, it is especially useful when NMR, X-ray, or other techniques are problematic or one simply needs a fast technique to verify the s

SUBMITTER: Micsonai A 

PROVIDER: S-EPMC9110821 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature