Analysis of conditional colocalization relationships and hierarchies in three-color microscopy images.

ABSTRACT: Colocalization analysis of multicolor microscopy images is a cornerstone approach in cell biology. It provides information on the localization of molecules within subcellular compartments and allows the interrogation of known molecular interactions in their cellular context. However, almost all colocalization analyses are designed for two-color images, limiting the type of information that they reveal. Here, we describe an approach, termed "conditional colocalization analysis," for analyzing the colocalization relationships between three molecular entities in three-color microscopy images. Going beyond the question of whether colocalization is present or not, it addresses the question of whether the colocalization between two entities is influenced, positively or negatively, by their coloc

SUBMITTER: Vega-Lugo J 

PROVIDER: S-EPMC9111757 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature